Lethal factor interacts with several MAPKK molecules as mentioned on the biological function page. Below I have discussed the interactions LF has with small molecules such as inhibitors.

Electron cloud density map of anthrax lethal factor with bound substrates shown with ball and stick models. The red patches indicate negative charge while the blue indicates positive charge. Image courtesy of http://www.nature.com/nsmb/journal/v11/n1/fig_tab/nsmb708_F2.html
Protein Screening, LF Inhibition
Edit
Turk et al. (2003) ran an extensive peptide screen on potential lethal factor substrates. 
Bond line diagram of GM6001, also known as galardin or ilomastat. Image from http://en.wikipedia.org/wiki/GM6001
Curcumin
Edit

Native curcumin (compound 1) along with four other chemically modified cur cumin derivatives. Photo courtesy of http://informahealthcare.com/doi/pdf/10.3109/14756366.2013.837901
Curcumin, the active ingredient of the eastern spice turmeric (Curcuma longa) popular in Asian countries is known to have many medicinal purposes. Becuase of its many functional groups, curcumin, in small amounts can act as an anti-inflammatory, protein kinase, and prion among other things. Antonelli et al. (2013) ran assays to test the effect of Curcumin on lethal factor inhibition. They tested five chemically modified curcumin derivatives and found that all compounds except compound two were able to inhibit lethal factors peptidolytic activity. Unlike the study done by Turk et al. (2003), the curcumin molecules were found to not only competitively inhibit lethal factor, but were also able to bind to allosteric sites (compounds one and three) and funciton as noncompetitive inhibitors as well.
Project Pages
Edit
- Patrick: Anthrax Toxin: Introduction
- Patrick: Anthrax Toxin: Biological Function
- Patrick: Anthrax Toxin: Biosynthesis
- Patrick: Anthrax Toxin: Gene Sequence
- Patrick: Anthrax Toxin: Amino Acid Sequence and Composition
- Patrick: Anthrax Toxin: Secondary and Tertiary Structure
- Patrick: Anthrax Toxin: Domains and Structural Motifs
- Patrick: Anthrax Toxin: Interactions with macromolecules and small molecules
- Patrick: Anthrax Toxin: Molecular biodiversity and evolution
- Patrick: Anthrax Toxin: PyMOL Images
- Patrick: Anthrax Toxin: Literature Review
- Patrick: Anthrax Toxin: Useful online resources
References
Edit
Antonelli, A., Zhang, Y., Golub, L., Johnson, F., Simon, S. 2013. Inhibition of anthrax lethal factor by curcumin and chemically modified curcumin derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry. Epub ahead of print.
Dalkas, G., Papakyriakou, A., Vlamis-Gardikas, A., Spyroulias, G. 2009. Insights into the anthrax lethal factor–substrate interaction and selectivity using docking and molecular dynamics simulations. Protein Science. 18 (8):1774-1785
Nestorovoch EM, Bezrukov SM. 2014. Designing Inhibitors of Anthrax Toxin. Informa Health Care. 9(3):299-318
Turk, B. et al. 2003. The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor.Nature Structural and Molecular Biology. 11(1):60-66.